sCellTisu™: Ambient Tissue Stabilization Medium for Single-Cell RNA-Seq
- Keeps biopsies ready for 72 hours at 15–25°C. No ice.
- No crosslinking: tissue digests normally, viability 88% or more.
- Stops stress genes (FOS, JUN) and locks RNA at RIN 8.0 or higher.
Research use only (RUO).
What is sCellTisu™?
sCellTisu™ is an ambient transport medium for solid human and murine tissue biopsies. It prepares them for single-cell RNA-seq, single-nucleus RNA-seq and spatial transcriptomics.
What problem does it solve?
- Ice gives hours: fresh tissue declines fast on wet ice, so multi-centre studies lose samples.
- Freezing kills cells: flash-frozen tissue lyses on thawing and only suits nuclei work.
- Formalin ruins digestion: crosslinked tissue will not dissociate into live cells.
sCellTisu™ halts transcription and RNases on contact, then washes out completely so tissue digests as normal.
Which samples can I use it with?
- Needle core biopsy (14G–18G)
- Surgical tissue (3 mm or thinner)
- Human solid tissue
- Murine solid tissue
Designed for solid tissue. Use 1:10 to 1:20 tissue to medium.
How long can I store or transport the sample?
Which tests or workflows does it support?
- scRNA-seq
- snRNA-seq
- Spatial transcriptomics (10x Visium)
- 10x Chromium (3′, 5′, Multiome)
- BD Rhapsody™
- Smart-seq3
Compare the data
Transport methods compared
| Parameter | Wet ice | Flash-frozen (LN2) | Formalin / FFPE | sCellTisu™ |
|---|---|---|---|---|
| Transit stability | Under 2–4 hours | Sublimation hazard at -80°C | Ambient storage | Ambient 15–25°C, 72 h |
| Matrix crosslinking | None | None | Heavy covalent methylene | Zero (fully reversible) |
| Enzymatic dissociation | High if fresh | Unusable (needs snRNA) | Severely refractory | Retained after wash |
| Single-cell viability | Rapid linear decline | Lysis on thawing | 0% viable cells | 88% or more after 72 h |
| Stress artifacts | High induction | Low (rapid freeze) | Severe adduct distortion | Instant metabolic lock |
Performance after 72 hours at 22°C
| Quality metric | Fresh tissue (0 h) | sCellTisu™ (72 h, 22°C) | Conventional dehydration reagent |
|---|---|---|---|
| Cell recovery (cells/mg) | 1.20 × 10⁵ | 1.14 × 10⁵ | 0.32 × 10⁵ |
| Post-wash viability (AO/PI) | 91.5% | 88.5% | 52.3% |
| RNA Integrity Number (RIN) | 9.2 | 8.6 | 6.1 |
| Ambient RNA leakage (SoupX) | 2.2% | 2.9% | 16.4% |
| Immediate early genes (FOS/JUN) | High induction (over 4.2-fold) | Basal (0.02-fold shift) | Variable induction |
Source: Ruhvenile® internal benchmark (PDS-SCT-01, v2.1).
Specifications
| Appearance | Clear, colourless, particulate-free sterile liquid |
|---|---|
| Working pH (22°C) | 7.35 ± 0.05 |
| Active stabiliser | Proprietary patent-pending, non-crosslinking matrix |
| Additives | BSA-free, animal-free, detergent-free, serum-free |
| Sterility | 0.22 µm filtered; intrinsically bacteriostatic and fungicidal |
| Ratio | 1:10 to 1:20 tissue to medium |
| Shelf life | 18 months from manufacture, sealed at 15–25°C |
Catalogue codes
| Catalogue no. | Product | Pack |
|---|---|---|
| RVN-SCT-1.5 | sCellTisu™ Ambient Stabilization Vials (1.5 mL) | 10 pre-filled leak-proof vials |
| RVN-SCT-3.0 | sCellTisu™ Ambient Stabilization Vials (3 mL) | 10 pre-filled leak-proof vials |
How do I use it?
- Collect: cut tissue to 3 mm or thinner (14G–18G needle core or surgical slice).
- Immerse: drop it into sCellTisu™ at 1:10 or more and invert 3–5 times.
- Ship: seal tightly and send at 15–25°C. Do not freeze or put on ice.
- Wash: two washes in cold 1× DPBS with BSA (200 × g, 2 minutes).
- Digest: use your usual cocktail plus Ca²⁺/Mg²⁺ at 37°C for 25–40 minutes.
- Load: strain at 40 µm, check viability and load the microfluidic cartridge.
How is it different from other Ruhvenile® and available products?
- Wet ice: window of under 2–4 hours. sCellTisu™ gives 72 hours at room temperature.
- Flash-freezing: needs liquid nitrogen or dry ice and gives lysis on thawing. No cold chain here.
- Formalin or FFPE: crosslinks tissue and kills cells. sCellTisu™ is non-crosslinking.
- Dehydrating or precipitating reagents: in our benchmark they gave 0.32 × 10⁵ cells/mg and 52% viability, against 1.14 × 10⁵ and 88.5%.
- GeneLokr™ and truGnom®: these preserve extracted nucleic acids. Use sCellTisu™ when you need live, intact cells for single-cell work. See GeneLokr™.
Differences are described from product design and Ruhvenile® internal data.
FAQs
Is sCellTisu™ for diagnostic use?
No. sCellTisu™ is supplied for research use only (RUO).
How long can biopsies stay in sCellTisu™ at room temperature?
Up to 72 hours at 15–25°C, with cell viability of 88% or more after washing.
Does sCellTisu™ crosslink tissue like formalin?
No. It is non-crosslinking, so tissue digests with standard dissociation enzymes and cells stay viable for single-cell partitioning.
Do I need to wash the tissue before dissociation?
Yes. Wash twice in cold 1× DPBS with BSA (200 × g for 2 minutes), then digest with your usual cocktail supplemented with Ca²⁺/Mg²⁺.
Which single-cell platforms is it designed for?
10x Genomics Chromium (3′, 5′, Multiome), BD Rhapsody™, Smart-seq3 and 10x Visium spatial arrays.
What tissue-to-medium ratio should I use?
Use 1:10 to 1:20, with pieces no thicker than 3 mm.
What is the shelf life?
18 months from the date of manufacture when stored sealed at 15–25°C.
Datasheet download
sCellTisu™ datasheet
Mechanism, benchmarks, two-phase protocol and ordering details in a 2-page PDF.
Related products
Request a quote for sCellTisu™
Tell us your tissue type, platform and study size. We reply with a quote and can arrange bulk packs. Dispatch within 3–5 working days.
Indigenous patented technology, discovered and developed in IndiaPatented and proprietary product of Ruhvenile® · Made in India